sprague-dawley rat meaning in English
斯普拉-道来大鼠
Examples
- Experiments were performed on adult male sprague - dawley rats weighing 230 - 270g
本实验采用健康成年雄性sd大鼠,体重2509士209 。 - Enkephalin ( enk ) is one of endogenous opiate like substance and could induce psychological euphoria and modulate function of learning and memory . however there has no report available about the role of enk in acquisition of sprague - dawley rats as materials
脑啡肽( enk )是内源性阿片样物质的一种,可参与情绪反应调节,引起精神欣快感,并对学习记忆具有调制作用。 - Methods : male sprague - dawley rats were decapitated . the thoracic aorta was removed and washed in cold krebs - henseleit solution , then cut into 2 - 3 mm in length . the endothelium of some rings was removed by rubbing with a metal thread
取雄性sprague - dawley大鼠的胸主动脉条上段,置于4的k - h液中,将血管剪成2 3mm长的主动脉环,部分血管环先用机械法去除血管内皮,迅速悬挂至离体灌流装置中。 - In this experiment , radio - immunoassay and hybridization in situ were applied to observe the insulinotropic activities of glp - 1 ( 7 - 36 ) nh2 and reveal the mechanisms underlying this process . methods : rat pancreases were removed from 3 - 5 day - old sprague - dawley rats and dissected into 0 . 5mm3 segments and islets were isolated by the collagenase digestion method of wangling et al . thoroughly washed islets and suspended in modified rpmi - 1640 medium supplemented with 10 % fetal bovine serum , and added to 50ml cell culture flasks
方法:胰岛的分离参照王玲等的方法,每次实验取新生3 - 5天sd大鼠,无菌条件下剖腹取出胰腺,剪切为0 . 5mm ~ 3的组织块, v型胶原酶消化30min后,离心洗涤,悬浮于完全培养基,接种入50ml培养瓶,于5 co _ 2 、 95空气条件下培养20h ,转板纯化,接种于96孔培养板培养24h ,按实验要求进行实验。